WebHydroxyl radical (OH) production in sunlit natural waters is known to be an important factor in pollutant degradation and nutrient cycling, and various probes have been developed to study this species in aquatic systems. ... Terephthalate (TPA) is a known probe for OH that produces a fluorescent product, hydroxyterephthalate (hTPA), upon ... WebApr 11, 2024 · Methods . Esculetin’s effects on the proliferation, migration and apoptosis of HUH7 and HCCLM3 cells were detected by using CCK8, crystal violet staining, wound healing, Transwell TM and Annexin V-FITC/PI. Flow cytometry, fluorescence staining, Western blot, T-AOC, DPPH radical scavenging assay, hydroxyl radical’s inhibitory …
Mitochondrially targeted fluorescent redox sensors - Interface …
WebApr 6, 2024 · Highly ROS are able to irreversibly cleave the ether linkage and switch fluorescence on by removing the PET quencher. MitoHR was shown to be most sensitive towards the hydroxyl radical, displaying a greater than 500-fold fluoresence increase, while MitoAR showed greatest sensitivity to hypochlorite, with a 1500-fold fluorescence increase. WebApparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci U S A. 1990;87:1620-1624 ... Fluorescent probe for the imaging of superoxide and peroxynitrite during drug-induced liver injury. Chem Sci. 2024;12:3921-3928 63. Sun W, Shi Y-D, Ding A-X, ... greensboro nc hilton
Dichlorodihydrofluorescein Diacetate - an overview
WebApr 10, 2024 · Meanwhile, the hydroxyl radicals generated by the catalytic reaction of V−CDs could induce oxidative damage in tumor cells for CDT, while showing less cytotoxicity and side effects in normal cells. ... Professor Zhang’s research team constructed pH- and ATP-responsive fluorescence probes based on the Forster resonance energy … WebFeb 4, 2014 · The developed fluorescent sensor exhibited high selectivity for (•)OH over other reactive oxygen species (ROS), reactive nitrogen species (RNS), metal ions, and other biological species, as well as high accuracy and sensitivity with low detection limit to ∼0.68 μM, which fulfills the requirements for detection of (•)OH in a biological system. Web4.3.1 Molecular probes used to measure ROS 2′,7′-Dichlorodihydrofluorescein diacetate (H2 DCFDA) is probably the most commonly used probe for measuring cellular H 2 O 2 ( McLennan & Degli Esposti, 2000 ). The cell-permeable H 2 DCFDA diffuses into cells and is deacetylated by cellular esterases to form 2′,7′-dichlorodihydrofluorescein (H 2 DCF). fmb youtube