Ctll 2 proliferation assay
WebBioactivity: The ED50 of this protein, as measured by CTLL-2 cell proliferation assay, is less than or equal to 4 ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 10e5 Units/mg. Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 29.4 kDa. WebCellGenix® GMP rh IL-2 has an activity of 8 – 32 × 106 IU/mg. The activity of GMP rh IL-2 was measured in a cell proliferation assay using the IL-2-dependent cell line CTLL-2. It was calibrated against Proleukin®. You can find the batch specific activity on the certificate of analysis (CoA).
Ctll 2 proliferation assay
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Web2. Add WST-1 reagent from CTLL-2 cell proliferation Kit 3. Incubate for 1 – 4 h 4. Measure absorbance at 420 – 480 nm 5. Analyze data Advantages at a glance: Easy to use o …
WebWhen maintained in continuous culture, however, the overall sensitivity of this cell line is highly variable. To overcome this problem, large batches of CTLL-2 were grown, frozen and tested for their ability to detect low levels of IL-2 (0.01 IU) in dose response assays. Batches meeting this criterion were stored in liquid nitrogen until required. WebDownload scientific diagram CTLL-2 cells proliferation assay of purified superagonist to evaluate its bioactivity. The EC 50 was calculated by the four-parameter fit logistic equation to be 30 ...
WebFeb 24, 2024 · The immunocytokines that were used for this assay are schematically depicted (the antibody moiety is depicted as empty symbols and the cytokine moieties as hatched areas) [a] the proliferation of CTLL-2 and the response of the CTLL-2 reporter cell line triggered by L19-IL2 [b] the production of interferon-γ by NK-92 cells and the … WebThe purified siIL-15 stimulates the proliferation of cytotoxic T lymphocytes line (CTLL)-2 and Kit 225 cells with EC50 values of 3.1 and 32.5 ng/mL, respectively.
WebThe bioactivity of the superagonist was characterized by CTLL-2 cells proliferation assay in vitro, showing its capability of stimulating the expansion of memory CD8 + T cells (cluster of differentiation) in mouse spleen.
WebApr 11, 2024 · Recombinant Mouse IL2 (Catalog # 402 ML) stimulates cell proliferation of the CTLL 2 mouse cytotoxic T cell line. The ED 50 for this effect is 0.1 0.4 ng/mL. 6'6 3$*(1 μg/lane of Recombinant Mouse IL2 was resolved with SDSPAGE under reducing (R) conditions and visualized by silver staining, how much should ice skates costWebTitle: CTLL-2 Proliferation Assay for Determination of Bioactivity of Recombinant Human Interleukin 2 (rhIL-2) UNCONTROLLED COPY FOR TRAINING AND REFERENCE … how do the students at reardan show they careWebThe biological activity of the recovered soluble rhIL-2 protein was determined in a proliferation assay using CTLL-2 cells. Human IL-2 purchased from Sigma-Aldrich Co. … how much should insulation costWebJun 2, 2012 · A bioassay for IL-2 using CTLL-2 cells. Supernatants of tumor cells (PCl-13) transduced with the gene for human IL-2 were collected and tested for the ability to … how do the stomata open and closeWebApplication CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. [ 1] [ 2] Culture Medium RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS). Subculture Routine Maintain cultures between 3-9 x 10 5 cells/ml; 5% CO 2; 37°C. how do the structures function similarlyWebFor example, Recombinant Human IL-2 can activate proliferation of IL-2 dependent cell lines, CTLL-2 and HT-2. The same lot of recombinant protein provides different ED 50 values depending on the cell line used for stimulation. In the CTLL-2 stimulation assay, the ED 50 is 0.26 ng/ml corresponding to a specific activity of 3.73 x10 6 units/mg. how much should interior designers sellWebTitle: CTLL-2 Cell Proliferation Assay for Determination of Bioactivity of IL-15 . This procedure is made available through federal funds from the National Cancer Institute, NIH, under contract . UNCONTROLLED COPY FOR TRAINING AND REFERENCE PURPOSES ONLY . 7.5.2 Using a 50 -300 multiple channel pipettes, add the Starting Std01 or sample how do the strengths and weaknesses help us